Poster: A Reliable, Efficient, and Matrix-Free Method to Generate Midbrain Organoids from Human Pluripotent Stem Cells

Leon Chew, MSc | Senior Scientist

Poster: Rapid, High-Efficiency Differentiation of Motor Neurons from Human Pluripotent Stem Cells

Jerry Wang, PhD | Senior Scientist

Poster: The Downstream Differentiation Potential of Human Induced Pluripotent Stem Cell-Derived Neural Progenitor Cells to Forebrain Neurons and Astrocytes

Alym Moosa, MSc | Scientist

Poster: In Vitro Modeling of Parkinson’s Disease Using Human Pluripotent Stem Cell-Derived Midbrain Neuron and Microglia Co-Culture with Alpha-Synuclein Fibrils

Noëmie Leblanc, MSc | Senior Research Associate

Poster: It Takes Two – Or Three: Comparing Human Pluripotent Stem Cell-Derived Glia-Neuron Co-Cultures to Neuron Monoculture Under Basal and Injury Conditions

Jerry Wang, PhD | Senior Scientist

Poster: A Human Pluripotent Stem Cell-Derived Organoid Model for Recapitulation of Central Nervous System (CNS) Barrier and Fluid Secretion Functions of the Choroid Plexus

Leon Chew, MSc | Senior Scientist

Poster: An In Vitro Assay to Determine the Neurotoxic Effects of Pharmacological Compounds

Kasandra McCormack, BSc | Technical Scientist

Innovation Showcase: How to Choose the Right Neural Cell Culture Model for Your Research Question

Erin Knock, PhD | Associate Director, Neural Biology

Examining the nervous system at a cellular and molecular level is foundational to interpreting anatomical and psychological studies from live subjects, and choosing the optimal model for your question is key to obtaining relevant results. This Innovation Showcase will review the use of in vitro model systems and offer specific examples of the types of questions that can be answered with each model. Cultured rodent primary neurons provide mature cell types for neurotoxicity studies; we will present an example using BrainPhys™ medium to support and characterize neuron activity. For human context in flexible workflows, we will provide examples using STEMCELL’s STEMdiff™ product line to generate human pluripotent stem cell-derived cell types, such as pure sensory neuron cultures for pain modeling. Applications of culture systems with increasing complexity, such as neuron-astrocyte-microglia co-cultures for injury modeling and gene-edited organoid cultures for developmental modeling, will also be discussed. Throughout this session, we will demonstrate how STEMCELL’s tools can help overcome technical barriers to human stem cell culture and differentiation and enable the culture of complex, multi-cellular constructs when they are relevant.