Lab Basics
Lab Basics
Whether you're new to the lab or need to refresh your knowledge, it can be helpful to have resources on the fundamental research techniques to refer to. Start here for an introduction to cell culture, basic cell separation methods, fundamental cell assays, or other key molecular biology techniques.
Featured Protocol
- Video Freeze Human Primary Cells with CryoStor® CS10
- Tech Tip Aggregate Passaging for ES and iPS Cell Maintenance
- Protocol How to Prepare and Plate Semi-Solid Methylcellulose Medium for Cell Culture
- Protocol How to Coat Cultureware with Vitronectin XF™ for Pluripotent Stem Cell Culture
- Tech Tip Recommendations for Coating Plates with Matrigel® for Pluripotent Stem Cell Culture
- Tech Tip Using Vitronectin XF™ Defined Matrix for Human Pluripotent Stem Cells
- Protocol How to Thaw Frozen Primary Cells
- Protocol How to Create a Hypoxic Environment for Cell Culture
- Protocol How to Cryopreserve Peripheral Blood Mononuclear Cells (PBMCs)
Mastered the basics? See more resources:
- Video How to Resuspend Magnetically Labeled Cells After EasySep™ Separation
- Video How to Aspirate an EasyPlate™ Isolation
- Video How to Correctly Pour Liquid from SepMate™ Tubes
- Video How to Prep Flask-Based Samples for EasySep™
- Protocol How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
- Protocol How to Reduce Cell Clumping in Single Cell Suspensions
- Protocol How to Remove Granulocytes from Old Blood Samples
- Protocol How to Generate a Buffy Coat
- Tech TIp Centrifugation Speed and Time for Cells
- Tech TIp Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation Procedures
- Tech Tip Reducing Platelet Contamination
- Tech Tip Choosing a Negative or Positive Selection Strategy
- Tech Tip Comparing Cell Separation Platforms from STEMCELL Technologies
Mastered the basics? See more resources:
- Protocol How to Count Cells with a Hemocytometer
- Protocol How to Count Cell Spheres in Suspension Culture
- Tech Tip Total Nucleated Cell and Viable Cell Counts
- Video Procedure for Setting Up the CFU Assay
- Tech Tip Refrigerate, Freeze, or Fix Cells for Flow Cytometry or Storage
- Protocol How to Characterize Extracellular Vesicles by Western Blotting
Mastered the basics? See more resources:
- Tech Tip Acquiring DNA or RNA from Positively Selected Cells
- Technical Bulletin Evaluation of Genome Editing
Mastered the basics? See more resources:
