EasySep™ Mouse TIL (CD45) Positive Selection Kit

Immunomagnetic positive selection kit

EasySep™ Mouse TIL (CD45) Positive Selection Kit

Immunomagnetic positive selection kit

From: 772 USD
Catalog #
100-0350_C
Immunomagnetic positive selection kit
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Product Advantages


  • Fast, easy to use, and column-free

  • Optimized for tumors and tissue samples with a low CD45 start frequency

  • Flexible protocols for higher purity or recovery

What's Included

  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component A, 0.25 mL
  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component B, 0.25 mL
  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component C, 1.5 mL
  • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
Products for Your Protocol

Overview

The EasySep™ Mouse TIL (CD45) Positive Selection Kit is designed to isolate highly purified CD45+ tumor infiltrating leukocytes (TILs) by immunomagnetic positive selection. This kit has been optimized for use on single-cell suspensions of solid mouse tumors with a low CD45+ start cell frequency, including tumors induced by implantation of 4T1, B16-F10, and CT26.WT cell lines into syngeneic mice. Due to the heterogeneity of mouse tumors, this kit may require optimization.

This kit targets CD45+ cells for positive selection with antibodies recognizing the CD45 surface marker. Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, and cell-based experiments.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
Leukocytes
Species
Mouse
Sample Source
Other, Tumor
Selection Method
Positive
Application
Cell Isolation
Area of Interest
Cancer, Immunology

Data Figures

Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension. Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension. Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension.

Figure 1. EasySep™ Mouse TIL (CD45) Positive Selection Kit

Tumors were induced by B16-F10, 4T1, or CT26.WT cell lines and dissociated into single-cell suspensions. CD45+ TILs were isolated from single-cell suspensions at various start concentrations using the purple EasySep™ Magnet. (A) Starting with a B16-F10 tumor single-cell suspension at 1 x 10^8 cells/mL, the purities of the start and final isolated fractions are 14.8% and 95.2%, respectively. (B) Starting with a 4T1 tumor single-cell suspension at 4 x 10^7 cells/mL, the purities of the start and final isolated fractions are 37.6% and 93.0%, respectively. (C) Starting with a CT26.WT tumor single-cell suspension at 2.5 x 10^7 cells/mL, the purities of the start and final isolated fractions are 32.3% and 84.6%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Catalog #
100-0350
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All
Language
English
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Safety Data Sheet 1
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100-0350
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All
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English
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Safety Data Sheet 2
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100-0350
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All
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English
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Safety Data Sheet 3
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100-0350
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English
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Safety Data Sheet 4
Catalog #
100-0350
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All
Language
English