EasySep™ Mouse TIL (CD45) Positive Selection Kit

Immunomagnetic positive selection kit

EasySep™ Mouse TIL (CD45) Positive Selection Kit

Immunomagnetic positive selection kit

From: 772 USD
Catalog #
(Select a product)
Immunomagnetic positive selection kit
Add to Wish List

Product Advantages


  • Fast, easy to use, and column-free

  • Optimized for tumors and tissue samples with a low CD45 start frequency

  • Flexible protocols for higher purity or recovery

What's Included

  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component A, 0.25 mL
  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component B, 0.25 mL
  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component C, 1.5 mL
  • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
Products for Your Protocol

Overview

The EasySep™ Mouse TIL (CD45) Positive Selection Kit is designed to isolate highly purified CD45+ tumor infiltrating leukocytes (TILs) by immunomagnetic positive selection. This kit has been optimized for use on single-cell suspensions of solid mouse tumors with a low CD45+ start cell frequency, including tumors induced by implantation of 4T1, B16-F10, and CT26.WT cell lines into syngeneic mice. Due to the heterogeneity of mouse tumors, this kit may require optimization.

This kit targets CD45+ cells for positive selection with antibodies recognizing the CD45 surface marker. Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, and cell-based experiments.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
Leukocytes
Species
Mouse
Sample Source
Other, Tumor
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Cancer, Immunology

Data Figures

Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension. Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension. Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension.

Figure 1. EasySep™ Mouse TIL (CD45) Positive Selection Kit

Tumors were induced by B16-F10, 4T1, or CT26.WT cell lines and dissociated into single-cell suspensions. CD45+ TILs were isolated from single-cell suspensions at various start concentrations using the purple EasySep™ Magnet. (A) Starting with a B16-F10 tumor single-cell suspension at 1 x 10^8 cells/mL, the purities of the start and final isolated fractions are 14.8% and 95.2%, respectively. (B) Starting with a 4T1 tumor single-cell suspension at 4 x 10^7 cells/mL, the purities of the start and final isolated fractions are 37.6% and 93.0%, respectively. (C) Starting with a CT26.WT tumor single-cell suspension at 2.5 x 10^7 cells/mL, the purities of the start and final isolated fractions are 32.3% and 84.6%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0350
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0350
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0350
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0350
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
100-0350
Lot #
All
Language
English