Starter Kit for MethoCult™ H4034 Optimum
Overview
Browse our Frequently Asked Questions (FAQs) on performing the CFU assay and explore its utility as part of the cell therapy workflow.
- Starter Kit for MethoCult™ H4034 Optimum (Catalog #04064)
- MethoCult™ H4034 Optimum, 24 x 3 mL tubes (Catalog #04044)
- Iscove's Modified Dulbecco's Medium (IMDM) with 2% Fetal Bovine Serum, 100 mL (Catalog #07700)
- Ammonium Chloride Solution, 100 mL (Catalog #07800)
- 35 mm Culture Dishes, 10/pack (Catalog #27100)
- 60 mm Gridded Scoring Dishes, 20/pack (Catalog #100-0085)
- Blunt-end Needles, 30/bag (Catalog #28130)
- 3 cc Syringes, 30/bag (Catalog #28230)
- Colony Atlas (Catalog #28700)
Scientific Resources
Product Documentation
Document Type | Product Name | Catalog # | Lot # | Language |
---|---|---|---|---|
Document Type
Product Information Sheet 1
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
18M97945 or lower |
Language
English |
Document Type
Product Information Sheet 2
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
18K96651 or lower |
Language
English |
Document Type
Product Information Sheet 3
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
18K96518 or lower |
Language
English |
Document Type
Product Information Sheet 4
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
All |
Language
English |
Document Type
Product Information Sheet 5
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
All |
Language
English |
Document Type
Product Information Sheet 6
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
18M94587 or higher |
Language
English |
Document Type
Product Information Sheet 7
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
19A98311 or higher |
Language
English |
Document Type
Product Information Sheet 8
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
18M97358 or higher |
Language
English |
Document Type
Product Information Sheet 9
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
All |
Language
English |
Document Type
Product Information Sheet 10
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
All |
Language
English |
Document Type
Product Information Sheet 11
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
All |
Language
English |
Document Type
Technical Manual
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 1
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 2
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 3
|
Product Name
Starter Kit for MethoCult™ H4034 Optimum
|
Catalog #
04064 |
Lot #
All |
Language
English |
Educational Materials (4)
Frequently Asked Question
Why use semi-solid media?
Why use methylcellulose-based media?
Is it necessary to add antibiotics to the media?
Is there anything I can do if my cultures appear contaminated?
Why can't I use a pipette to dispense methylcellulose-based media?
Can I 'pluck' the colonies for individual analysis?
Why are low adherence dishes so important?
Can MethoCult™ products be used for lymphoid progenitor CFU assays?
Is it possible to set up CFU assays in a 24-well plate?
Can I stain colonies in MethoCult™ medium?
Are there differences in colony morphology with serum-free media?
Can MethoCult™ be made with alternate base media?
Is there a MethoCult™ formulation suitable for HPP-CFC (high proliferative potential colony forming cell)?
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Figure 1. Procedure Summary for Hematopoietic CFU Assays

Figure 2. Examples of Colonies Derived from CFU-GM in MethoCult™ H4034 Optimum

Figure 3. Examples of Colonies Derived from BFU-E in MethoCult™ H4034 Optimum
Publications (9)
Abstract
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.