hPSC Genetic Analysis Kit

qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells
hPSC Genetic Analysis Kit

qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells

1 Kit
Catalog # 07550
624 USD
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Required Products
  1. Genomic DNA Purification Kit
    Genomic DNA Purification Kit

    For purification of genomic DNA from cells or tissue

Overview

Quickly and cost-effectively detect the 8 most common karyotypic abnormalities reported in human pluripotent stem cells (hPSCs).

This qPCR-based kit enables the genetic screening of multiple hPSC lines and contains enough material to analyze 20 individual samples in triplicate. It uses double-quenched probes with a 5-carboxyfluorescein (5-FAM) dye to give superior performance over other, single-quenched probes. The kit includes qPCR Master Mix, ROX Reference Dye, Buffer, and primer/probe mixes designed to detect the critical minimal regions of the 8 most commonly mutated regions, as well as a primer/probe mix to a control location in the genome. A genomic DNA control is also provided with demonstrated normal copy number over the regions of interest.

To understand your results, input qPCR data into our online Genetic Analysis App, which will perform statistical analyses, assist with data interpretation, and provide visual representation of the data.
Components
• qPCR Master Mix (2X), 3 mL
• ROX Reference Dye, 0.2 mL
• Chr 1q Genetic Assay, 60 Rxn
• Chr 4p Genetic Assay, 60 Rxn
• Chr 8q Genetic Assay, 60 Rxn
• Chr 10p Genetic Assay, 60 Rxn
• Chr 12p Genetic Assay, 60 Rxn
• Chr 17q Genetic Assay, 60 Rxn
• Chr 18q Genetic Assay, 60 Rxn
• Chr 20q Genetic Assay, 60 Rxn
• Chr Xp Genetic Assay, 60 Rxn
• Genomic DNA Control, 15 µL
• TE Buffer, 1 mL
Cell Type
Pluripotent Stem Cells
Species
Human
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 10
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 11
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 12
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 13
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English

Educational Materials (31)

Brochure
Detect Karyotypic Abnormalities in Human ES & iPS Cells
Technical Bulletin
hPSC Genetic Analysis Kit Technical Tips & Tricks
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
Video
How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
3:35
How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
Video
How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free Media
1:29
How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free Media
Video
How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
5:20
How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
Video
How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
7:57
How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
Video
How to Set Up an Assay with the hPSC Genetic Analysis Kit Experiment
8:33
How to Set Up an Assay with the hPSC Genetic Analysis Kit Experiment
Webinar
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
50:49
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
Webinar
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank
21:53
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank
Webinar
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells
24:46
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells
Webinar
Nature Research Round Table: Genomic Integrity of Human Pluripotent Stem Cells
15:47
Nature Research Round Table: Genomic Integrity of Human Pluripotent Stem Cells
Webinar
Nature Research Round Table: Identifying Acquired and Background Genetic Variants in Human Pluripotent Stem Cells
16:56
Nature Research Round Table: Identifying Acquired and Background Genetic Variants in Human Pluripotent Stem Cells
Webinar
Survey Results: Insights and Trends in Pluripotent Stem Cell Research
13:58
Survey Results: Insights and Trends in Pluripotent Stem Cell Research
Webinar
Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration
21:35
Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration
Webinar
CRISPR-Cas9 Gene Editing of Cerebral Organoids to Model Microcephaly
10:08
CRISPR-Cas9 Gene Editing of Cerebral Organoids to Model Microcephaly
Webinar
Considerations for High-Efficiency Genome Editing of Human Pluripotent Stem Cells
50:01
Considerations for High-Efficiency Genome Editing of Human Pluripotent Stem Cells
Webinar
Maintaining and Assessing High-Quality hPSC Cultures
55:53
Maintaining and Assessing High-Quality hPSC Cultures
Webinar
Nature Research Round Table: Human Pluripotent Stem Cell Lines as Disease Models
17:30
Nature Research Round Table: Human Pluripotent Stem Cell Lines as Disease Models
Webinar
Nature Research Round Table: Best Practices for the QC of Genome-Edited hPSC Lines - Panel Discussion
31:25
Nature Research Round Table: Best Practices for the QC of Genome-Edited hPSC Lines - Panel Discussion
Webinar
Nature Research Round Table: Genome Editing in Human Pluripotent Stem Cells
17:45
Nature Research Round Table: Genome Editing in Human Pluripotent Stem Cells
Webinar
Nature Research Round Table: HLA Typing Considerations for Human Pluripotent Stem Cell Banking
13:01
Nature Research Round Table: HLA Typing Considerations for Human Pluripotent Stem Cell Banking
Webinar
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenance of Human Pluripotent Stem Cells (hPSCs)
42:10
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenance of Human Pluripotent Stem Cells (hPSCs)
Webinar
Quality Control Guidelines for Clinical-Grade Human Induced Pluripotent Stem Cell Lines
1:13:44
Quality Control Guidelines for Clinical-Grade Human Induced Pluripotent Stem Cell Lines
Webinar
Re-Creating Disease with Kidney Organoids and CRISPR
1:14:36
Re-Creating Disease with Kidney Organoids and CRISPR
Webinar
Nature Research Round Table: hPSC Lines for Cell Therapies - Panel Discussion
29:03
Nature Research Round Table: hPSC Lines for Cell Therapies - Panel Discussion
Webinar
Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells
19:35
Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells
Webinar
Nature Research Round Table: The Process of Human Pluripotent Stem Cell Adaptation
18:48
Nature Research Round Table: The Process of Human Pluripotent Stem Cell Adaptation
Webinar
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
13:02
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
Webinar
Human Pluripotent Stem Cells for the Treatment of Age-Related Macular Degeneration and Compliance Considerations for Clinical-Grade iPSCs
47:23
Human Pluripotent Stem Cells for the Treatment of Age-Related Macular Degeneration and Compliance Considerations for Clinical-Grade iPSCs
Webinar
Nature Research Round Table: Pluripotency Tests
17:46
Nature Research Round Table: Pluripotency Tests
Load More Educational Materials

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. The hPSC Genetic Analysis Kit Identifies Chromosome 12 Trisomy

Chromosome 12 trisomy in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.

Figure 2. The hPSC Genetic Analysis Kit Identifies Chromosome 1 Duplication via Unbalanced Translocation

Unbalanced rearrangement of chromosome 1 in the WLS-1C human iPS cell line in which an extra copy of the long (q) arm of chromosome 1 translocated to the short arm (p) of chromosome 21 was (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.

Figure 3. The hPSC Genetic Analysis Kit Identifies Chromosome 20q11.21 Duplication

Chromosome 20q duplication in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar), (B) undetected by G-banding, and (C) confirmed by fluorescent in situ hybridization using probes for 20p11 (green) and 20q11.21 (red).

Figure 4. The hPSC Genetic Analysis Kit Identifies Abnormalities in Cultures with Approximately 30% Mosaicism

Genetically normal WLS-1C human iPS cells were mixed in the indicated ratios with WLS-1C human iPS cells containing a chromosome 20q duplication. Cultures with approximately 30% genetically abnormal cells exhibit a significantly enriched population of 20q11.21 duplication (orange bars).

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.