EasySep™ Release Mouse APC Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.

EasySep™ Release Mouse APC Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

From: 862 USD
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Immunomagnetic positive selection kit using particle release technology
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Product Advantages


  • Highly purified cells labeled with APC-conjugated antibodies isolated from mouse tissue in less than 40 minutes

  • No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

  • EasySep™ Release Mouse APC Positive Selection Kit (Catalog #100-0033)
    • EasySep™ Release APC Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
    • EasySep™ Mouse FcR Blocker (Catalog #18731), 0.5 mL

Overview

The EasySep™ Release Mouse APC Positive Selection Kit is designed to isolate cells that are labeled with APC-conjugated antibodies from mouse tissue by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, APC-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Subtype
Cell Isolation Kits
Cell Type
Other
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Using the EasySep™ Release Mouse APC Positive Selection Kit, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.

Figure 1. Purity of CD45+ Cells Following Cell Isolation with the EasySep™ Release Mouse APC Positive Selection Kit

In the above example, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0033
Lot #
1000157896 or lower
Language
English
Catalog #
100-0033
Lot #
1000157897 or higher
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Catalog #
100-0033
Lot #
All
Language
English
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.