EasySep™ Release Mouse APC Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology
EasySep™ Release Mouse APC Positive Selection Kit

Immunomagnetic positive selection of particle-free mouse cells labeled with APC-conjugated antibodies

1 x 10^9 cells
Catalog # 100-0033
746 USD
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Release Mouse APC Positive Selection Kit is designed to isolate cells that are labeled with APC-conjugated antibodies from mouse tissue by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, APC-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Advantages
• Highly purified cells labeled with APC-conjugated antibodies isolated from mouse tissue in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components
  • EasySep™ Release Mouse APC Positive Selection Kit (Catalog #100-0033)
    • EasySep™ Release APC Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
    • Normal Rat Serum, 2 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Subtype
Cell Isolation Kits
Cell Type
Other
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English

Educational Materials (4)

Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Scientific Poster
Fast and Easy Immunomagnetic Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable RapidSpheres™ Results in Functional, Pure and Particle-Free Cells
Scientific Poster
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres™
Scientific Poster
Releasable RapidSpheres Enable Immunomagnetic Purification of Highly Viable and Functional Immune Cells from Complex Tissues in Less Than 30 Minutes

Data and Publications

Data

Using the EasySep™ Release Mouse APC Positive Selection Kit, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.

Figure 1. Purity of CD45+ Cells Following Cell Isolation with the EasySep™ Release Mouse APC Positive Selection Kit

In the above example, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.

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