Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells
Cystic Fibrosis (CF) is a genetic disease that affects multiple organs in the body. The primary defects are in the airway and related to epithelial dysfunction. Technological advances have enabled researchers to study CF using specialized in vitro cell culture models to recapitulate the pseudostratified airway epithelium. In this webinar, Dr. Theo Moraes elaborates on the advantages of using nasal epithelial cell cultures to perform such studies and their implications for precision medicine in CF. He also discusses the Program for Individualized Cystic Fibrosis Therapy (CFIT)—a collaboration between CF Canada, SickKids Foundation, and The Hospital for Sick Children—and how it utilizes the nasal culture methodology to develop a nationally accessible resource and to accelerate the development of individualized therapies for CF patients.
Dr. Theo Moraes is a clinician and researcher at The Hospital for Sick Children and the SickKids Research Institute in Toronto, Canada.
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Serum- and BPE-free medium for expansion of primary human airway epithelial cells -
CELLTREAT® 0.4 µm Polyethylene Membrane Inserts
Polystyrene plate with lid and polyethylene membrane inserts; 6-, 12-, 24-well formats -
Serum- and BPE-free medium for human airway epithelial cells cultured at the air-liquid interface -
HTS Transwell®-96, 0.4 µm Pore Polyester Membrane Inserts
96-well polystyrene plates with two lids and polyester membrane inserts
