MethoCult™ SF H4636 Without EPO

Serum-free methylcellulose-based medium with recombinant cytokines for human hematopoietic cells

MethoCult™ SF H4636 Without EPO

Serum-free methylcellulose-based medium with recombinant cytokines for human hematopoietic cells

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Serum-free methylcellulose-based medium with recombinant cytokines for human hematopoietic cells
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Overview

MethoCult™ SF H4636 Without EPO is a complete methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow (BM), mobilized peripheral blood (MPB), peripheral blood (PB), and cord blood (CB) samples. MethoCult™ SF H4636 Without EPO is formulated to support the optimal growth of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M). It is suitable for use with CD34+ enriched cells, mononuclear cells, and cells isolated by other purification methods.

Browse our Frequently Asked Questions (FAQs)on performing the CFU assay.
Contains
• Methylcellulose in Iscove's IMDM
• Bovine serum albumin
• 2-Mercaptoethanol
• Recombinant human (rh) insulin
• Human transferrin (iron-saturated)
• rh stem cell factor (SCF)
• rh interleukin 3 (IL-3)
• rh granulocyte colony-stimulating factor (G-CSF)
• rh granulocyte-macrophage colony-stimulating factor (GM-CSF)
• Supplements
Subtype
Semi-Solid Media, Specialized Media
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Application
Cell Culture, Colony Assay, Functional Assay
Brand
MethoCult
Area of Interest
Stem Cell Biology
Formulation Category
Serum-Free

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0945
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0945
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.