ImmunoCult™ Human B Cell Expansion Kit

Serum-free culture kit for expansion of B cells
ImmunoCult™ Human B Cell Expansion Kit

Serum-free culture kit for expansion of human B cells

1 Kit
Catalog # 100-0645
995 USD
ImmunoCult™-XF B Cell Base Medium

Serum-free and xeno-free base medium for the expansion of human B cells

100 mL
Catalog # 100-0646
94 USD
ImmunoCult™-ACF Human B Cell Expansion Supplement

Serum-free and animal component-free culture supplement for expansion of human B cells

2 mL
Catalog # 10974
996 USD

Overview

ImmunoCult™ Human B Cell Expansion Kit comprises ImmunoCult™-XF B Cell Base Medium and ImmunoCult™-ACF Human B Cell Expansion Supplement, which together ensure consistent activation and expansion of human B cells and their maturation to plasma cells. B cells can be harvested and used directly in downstream applications after just one week or less in culture.
Advantages
⦁ Robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates
⦁ Serum-free medium and animal component-free supplement
Components
  • ImmunoCult™-XF B Cell Base Medium, 100 mL
  • ImmunoCult™-ACF Human B Cell Expansion Supplement, 2 mL
Subtype
Specialized Media, Supplements
Cell Type
B Cells
Species
Human
Application
Cell Culture
Brand
ImmunoCult
Area of Interest
Antibody Development, Cell Therapy, Drug Discovery and Toxicity Testing, Immunology, Infectious Diseases, Stem Cell Biology
Formulation
Serum-Free, Xeno-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Human B Cell Expansion Kit
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
ImmunoCult™-XF B Cell Base Medium
Catalog #
100-0646
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ImmunoCult™ Human B Cell Expansion Kit
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ImmunoCult™ Human B Cell Expansion Kit
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™-XF B Cell Base Medium
Catalog #
100-0646
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English

Educational Materials (7)

Brochure
Tools For Your Immunology Research
Brochure
Isolate Human Immune Cells
Wallchart
Antigen Processing and Presentation
Wallchart
The Immune Response to HIV Poster
Webinar
Online Immunology Journal Club: The Potential of Antibody-Producing B Cells for Modelling and Therapy
34:58
Online Immunology Journal Club: The Potential of Antibody-Producing B Cells for Modelling and Therapy
Webinar
Online Immunology Journal Club: Advances in Live-Attenuated Vaccine Development for Zika Virus
28:44
Online Immunology Journal Club: Advances in Live-Attenuated Vaccine Development for Zika Virus
Scientific Poster
Robust Serum-Free Expansion of Human B Cells In Vitro with an Animal Component-Free Cell Culture Supplement

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Fold Expansion and Maturation of B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

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