ImmunoCult™ Human B Cell Expansion Kit

Serum-free culture kit for expansion of B cells

ImmunoCult™ Human B Cell Expansion Kit

Serum-free culture kit for expansion of B cells

ImmunoCult™ Human B Cell Expansion Kit
1 Kit
1,085 USD
Catalog # 100-0645

Serum-free culture kit for expansion of human B cells

INDIVIDUAL COMPONENTS

ImmunoCult™-XF B Cell Base Medium

102 USD
Catalog # 100-0646

Serum-free and xeno-free base medium for the expansion of B cells

100 mL

ImmunoCult™-ACF Human B Cell Expansion Supplement

1,064 USD
Catalog # 10974

Serum-free and animal component-free culture supplement for expansion of human B cells

2 mL

Product Advantages


  • Robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates

  • Serum-free medium and animal component-free supplement

What's Included

  • ImmunoCult™-XF B Cell Base Medium, 100 mL
  • ImmunoCult™-ACF Human B Cell Expansion Supplement, 2 mL

Overview

Reliably expand human B cells that are ready for use in downstream assays and applications—without the use of serum, specialized cultureware, or problematic feeder cells.

ImmunoCult™ Human B Cell Expansion Kit comprises ImmunoCult™-ACF Human B Cell Expansion Supplement (Catalog #10974) and ImmunoCult™-XF B Cell Base Medium (Catalog #100-0646), which together ensure consistent activation and expansion of human B cells and their maturation to plasma cells. B cells can be harvested and used directly in downstream applications after just one week or less in culture.

This kit is compatible with many of our other upstream and downstream products. For example, you can use EasySep™ cell separation kits to isolate a range of B cell subsets—such as pan-B cells, memory B cells, and naïve B cells—that can be isolated and then immediately expanded using ImmunoCult™ Human B Cell Expansion Kit.
Subtype
Specialized Media, Supplements
Cell Type
B Cells
Species
Human
Application
Cell Culture
Brand
ImmunoCult
Area of Interest
Antibody Development, Cell Therapy Research, Drug Discovery and Toxicity Testing, Immunology, Infectious Diseases, Stem Cell Biology
Formulation
Serum-Free, Xeno-Free

Data Figures

Fold Expansion and Maturation of B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0645
Lot #
All
Language
English
Catalog #
100-0645
Lot #
All
Language
English
Catalog #
100-0646
Lot #
All
Language
English
Catalog #
100-0646
Lot #
All
Language
English
Catalog #
10974
Lot #
All
Language
English
Catalog #
10974
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0646
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
10974
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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