ArciTect™ tracrRNA Kit

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing
Catalog #
76016_C
Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing
From: 103 USD
Required Products
  1. ArciTect™ Cas9 Nuclease
    ArciTect™ Cas9 Nuclease

    Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing

  2. ArciTect™ crRNA
    ArciTect™ crRNA

    Custom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing

  3. Nuclease-Free Water|79001
    Nuclease-Free Water

    DNase- and RNase-free water for molecular biology applications

Overview

ArciTect™ tracrRNA is a trans-activating CRISPR RNA (crRNA), one of two RNA components required to make a guide RNA (gRNA) for CRISPR-Cas9 genome editing. ArciTect™ tracrRNA contains a complementary sequence to the linker sequence in ArciTect™ crRNA, allowing fast and efficient gRNA duplex formation. ArciTect™ tracrRNA is recognized by all ArciTect™ Cas9 nucleases and is essential for ribonucleoprotein (RNP) complex formation. It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency.
The ArciTect™ tracrRNA Kit includes tracrRNA as well as ArciTect™ Annealing Buffer (5X), which is required for forming a gRNA duplex between ArciTect™ tracrRNA and ArciTect™ crRNA. ArciTect™ crRNA is available by custom order for user-specified target sequences, in 2 nmol (Catalog #76010), 10 nmol (Catalog #76011), and 20 nmol (Catalog #76012) sizes. ArciTect™ Cas9 Nuclease is also available separately (Catalog #76001).
Components
  • ArciTect™ tracrRNA Kit, 5 nmol (Catalog #76016)
    •  ArciTect™ tracrRNA, 5 nmol
    • ArciTect™ Annealing Buffer (5X), 100 µL
 
  • ArciTect™ tracrRNA Kit, 10 nmol (Catalog #76017)
    • ArciTect™ tracrRNA, 10 nmol
    • ArciTect™ Annealing Buffer (5X), 100 µL
 
  • ArciTect™ tracrRNA Kit, 20 nmol (Catalog #76018)
    • ArciTect™ tracrRNA, 20 nmol
    • ArciTect™ Annealing Buffer (5X), 100 µL
Cell Type
Pluripotent Stem Cells
Species
Human
Brand
ArciTect
Area of Interest
Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ArciTect™ tracrRNA Kit
Catalog #
76016, 76017, 76018
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ArciTect™ tracrRNA Kit
Catalog #
76016, 76017, 76018
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ArciTect™ tracrRNA Kit
Catalog #
76016, 76017, 76018
Lot #
All
Language
English

Educational Materials (6)

Brochure
ArciTect™ CRISPR-Cas9 Genome Editing System
Technical Bulletin
Genome Editing of Human Primary T Cells Using CRISPR-Cas9
Technical Bulletin
Genome Editing of Human Pluripotent Stem Cells Using the ArciTect™ CRISPR-Cas9 System
Technical Bulletin
Genome Editing with Direct Cas9 RNP Delivery Design Considerations
Technical Bulletin
Evaluation of Genome Editing
Webinar
Improving Genome Editing with Enhanced CRISPR-Cas Nucleases
57:20
Improving Genome Editing with Enhanced CRISPR-Cas Nucleases

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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