ArciTect™ crRNA

Custom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing
Catalog #
76010_C
Custom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing
From: 103 USD
Design Guides
Required Products
  1. ArciTect™ Cas9 Nuclease
    ArciTect™ Cas9 Nuclease

    Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing

  2. ArciTect™ tracrRNA Kit
    ArciTect™ tracrRNA Kit

    Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing

Overview

ArciTect™ crRNA is a custom CRISPR RNA (crRNA), one of two synthetic RNA components required to make a guide RNA (gRNA) template for CRISPR-Cas9 genome editing. ArciTect™ crRNA contains a user-specified 19 - 21 base sequence complementary to the genomic location to be cleaved, and a linker sequence complementary to ArciTect™ tracrRNA (Catalog #76016), allowing fast and efficient gRNA duplex formation. ArciTect™ crRNA should be designed so that it is directly upstream of a protospacer adjacent motif (PAM) site (NGG). It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency.
Cell Type
Pluripotent Stem Cells
Species
Human
Brand
ArciTect
Area of Interest
Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ArciTect™ crRNA
Catalog #
76010, 76011, 76012
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ArciTect™ crRNA
Catalog #
76010, 76011, 76012
Lot #
All
Language
English

Educational Materials (9)

Brochure
ArciTect™ CRISPR-Cas9 Genome Editing System
Technical Bulletin
Genome Editing with Direct Cas9 RNP Delivery Design Considerations
Technical Bulletin
Evaluation of Genome Editing
Technical Bulletin
Genome Editing of Human Primary T Cells Using CRISPR-Cas9
Technical Bulletin
Genome Editing of Human Pluripotent Stem Cells Using the ArciTect™ CRISPR-Cas9 System
Webinar
Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types
1:07:14
Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types
Webinar
CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells
55:51
CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells
Webinar
Improving Genome Editing with Enhanced CRISPR-Cas Nucleases
57:20
Improving Genome Editing with Enhanced CRISPR-Cas Nucleases
Scientific Poster
Optimization of Human T Cell Activation and Expansion Protocols Improves Efficiency of Genetic Modification and Overall Cell Yield

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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