ImmunoCult™ Mouse B Cell Expansion Kit

Serum-free culture kit for in vitro expansion of mouse B cells.

ImmunoCult™ Mouse B Cell Expansion Kit

Serum-free culture kit for in vitro expansion of mouse B cells.

From: 895 USD
Catalog #
100-1003_C
Serum-free culture kit for in vitro expansion of mouse B cells.

Product Advantages

⦁ Achieve robust in vitro expansion of mouse B cells without the use of serum, feeder cells, or specialized culture plates
⦁ Simplify your workflow, with an optimized and easy-to-use culture system
⦁ Obtain consistent yields of expanded mouse B cells

What's Included

  • ImmunoCult™-XF B Cell Base Medium, 100 mL (Catalog #100-0646)
  • ImmunoCult™-ACF Mouse B Cell Expansion Supplement, 2 mL (Catalog #100-1004)

Overview

Consistently expand mouse B cells in serum-free conditions without the use of problematic feeder cells.

Use ImmunoCult™ Mouse B Cell Expansion Kit to provide optimized culture conditions for expanding B cells from mouse splenocytes with high yields. This kit comprises ImmunoCult™-XF B Cell Base Medium and ImmunoCult™ACF Mouse B Cell Expansion Supplement, providing you with a robust and easy-to-use culture system. After just 6 - 9 days in culture, cells can be harvested and used directly in your downstream applications.

This kit is compatible with our other upstream and downstream products. For example, you can isolate B cells with EasySep™ Mouse Pan-B Cell Isolation Kit and expand them immediately using ImmunoCult™ Mouse B Cell Expansion Kit.
Subtype
Specialized Media
Cell Type
B Cells
Species
Mouse
Application
Cell Culture, Expansion
Brand
ImmunoCult
Area of Interest
Antibody Development, Cell Therapy Research, Immunology, Infectious Diseases
Formulation
Animal Component-Free, Serum-Free, Xeno-Free

Data Figures

Protocol Diagram for Culturing Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

Figure 1. Protocol Diagram for Culturing Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured in complete Mouse B Cell Expansion Medium as described in Directions For Use (steps 1 - 7). B Cells were harvested on day 9 for analysis. Cells can also be harvested at earlier time points depending on different applications.

Expansion of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

Figure 2. Expansion of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Fold expansion of viable cells is shown with bar graphs representing the mean ± SEM (n = 8). B cells expanded 176.9 ± 29.8-fold after 9 days of culture.

Maturation of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

Figure 3. Maturation of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Following staining using the protocol by Pracht et al. (Eur J Immunol, 2017), the expression of A) B220 and CD138 and B) TACI (CD267) and CD86 were analyzed by flow cytometry at several time points (data represents mean ± SEM, n = 8). An increase in CD86 cell surface expression indicates B cell activation; a decrease in B220 and an increase in CD138 and TACI cell surface expression indicate maturation of B cells to plasmablasts or plasma cells.

Growth of Mouse B Cells When Cultured with ImmunoCult™ Mouse B Cell Expansion Kit Over the Span of 9 Days

Figure 4. Robust Growth of Mouse B Cells when Cultured with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Cells were imaged at A) 10X magnification on day 0, B) 10X magnification on day 3, C) 40X magnification on day 6, and D) 40X magnification on day 9.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1003
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-1003
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-1003
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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