STEMdiff™ Endothelial Expansion Culture Kit

Expand human pluripotent stem cell-derived endothelial cells

STEMdiff™ Endothelial Expansion Culture Kit

Expand human pluripotent stem cell-derived endothelial cells

From: 436 USD
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Expand human pluripotent stem cell-derived endothelial cells
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What's Included

  • STEMdiff™ Endothelial Expansion Culture Kit (Catalog #100-1218)
    • STEMdiff™ Endothelial Expansion Basal Medium, 120 mL
    • STEMdiff™ Endothelial Expansion 5X Supplement, 30 mL
    • Animal Component-Free Cell Attachment Substrate, 1 mL

Overview

Efficiently expand human pluripotent stem cell (hPSC)-derived endothelial cells using xeno-free STEMdiff™ Endothelial Expansion Culture Kit (Catalog #100-1218). This kit includes xeno-free STEMdiff™ Endothelial Expansion Basal Medium and 5X Supplement, and Animal Component-Free Cell Attachment Substrate, and is is optimized for the expansion of hPSC-derived endothelial cells and for the maintenance of endothelial marker expression. After 7 days of mesoderm and endothelial induction, the resulting cells can be expanded for 5 - 6 passages to generate large numbers of highly pure, mature endothelial cells that typically reach ≥ 99% CD144+CD31+ by passage 2 and maintain this phenotype until passage 5.

This kit is compatible with hPSCs maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), or TeSR™-E8™ (Catalog #05990) on Corning® Matrigel®. The components of STEMdiff™ Endothelial Expansion Culture Kit are included in STEMdiff™ Endothelial Differentiation Kit (Catalog #08005). For passaging, Animal Component-Free Cell Dissociation Kit (Catalog #05426) is required.



NOTE: Heparin Solution (Catalog #07980) is required for preparation of complete STEMdiff™ Endothelial Expansion Medium.



CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.



This product may be used for research purposes only. Please contact STEMCELL for any clinical or commercial application of the Product.
Cell Type
Endothelial Cells
Application
Cell Culture, Expansion
Brand
STEMdiff
Area of Interest
Angiogenic Cell Research, Disease Modeling, Endothelial Cell Biology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1218
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-1218
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-1218
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.