PBS-MINI MagDrive Bioreactor
Overview
PBS-MINI 0.1 L and 0.5 L MAG Single-Use Vessels are sold separately in packs of 4. These disposable vessels are animal component-free and meet the requirements for USP Class VI Testing for Plastics <88> and ISO 10993.
The PBS-MINI MagDrive Bioreactor Base Unit includes a power adapter that is compatible with Type A electrical outlets and 120V or 240V A/C inputs. A separate wall adaptor is needed for use outside North America. An 18-month warranty is provided by PBS Biotech Inc., valid from the purchase date. This product is for Research Use Only.
For more information, contact techsupport@stemcell.com.
⦁ SAFE & EFFICIENT EXPANSION. The Vertical-Wheel™ impeller and U-shaped base create a low-shear mixing environment that is optimal for culturing hPSC aggregates of uniform size.
⦁ EASY AGGREGATE RECOVERY. The U-shaped base collects aggregates and the wide diameter cap provides easy access for aggregate recovery.
⦁ A PERFECT PAIR. The Base Unit is designed to be used with the 0.1 L and 0.5 L PBS-MINI MAG Single-Use Vessels, magnetically driving the vessels via MagDrive technology.
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
PBS-MINI MagDrive Bioreactor Base Unit
|
Catalog #
100-1005 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
PBS-MINI 0.1 MAG Single-Use Vessel
|
Catalog #
100-1006 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
PBS-MINI 0.5 MAG Single-Use Vessel
|
Catalog #
100-1007 |
Lot #
All |
Language
English |
|
Document Type
Technical Manual
|
Product Name
PBS-MINI MagDrive Bioreactor
|
Catalog #
100-1005 |
Lot #
All |
Language
English |
Educational Materials (7)
Data and Publications
Data
Figure 1. Consistently High Daily Fold Expansion of Human Pluripotent Stem Cells (hPSCs) Is Achieved with the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels
Daily fold expansions of a human embryonic stem (ES) cell line (H9) and human induced pluripotent stem (iPS) cell line (WLS-1C), cultured in PBS-MINI (A) 0.1 and (B) 0.5 MAG Single-Use Vessels, show consistently high growth. The cells were seeded at a density of 8 x 104 viable cells/mL in 100 mL (for PBS-MINI 0.1 MAG) and 3 - 8 x 104 viable cells/mL in 500 mL (for PBS-MINI 0.5 MAG), and cultured for 4 days using mTeSR™3D (Catalog #03950). Fold expansion of each cell line was comparable to their growth in Nalgene™ filter bottles (Control), when seeded at a density of 1 x 105 viable cells/mL in 30 mL. Error bars are +/- SD (n = 3 for PBS-MINI 0.1 and 0.5 conditions, and n = 2 for control).
Figure 2. Culturing hPSCs in the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels Achieves High Cell Viability
End-of-passage viabilities of a human ES cell line (H9) and human iPS cell line (WLS-1C) cultured in mTeSR™3D show that the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels support expansion of hPSCs at (A) 100 mL (with PBS-MINI 0.1 MAG) and (B) 500 mL (with PBS-MINI 0.5 MAG) culture volumes. Error bars are +/- SD (n = 3 for PBS-MINI 0.1 and 0.5 conditions, and n = 2 for control).
Figure 3. Markers of Undifferentiated hPSCs Harvested from the PBS-MINI 0.5 MAG Single-Use Vessels
1C and H9 hPSC lines after 9 passages in the PBS-MINI 0.5 MAG Single-Use Vessels using mTeSR™3D show high OCT-4 and TRA-1-60 expression. Error bars are +/- SD (n = 1).
Figure 4. Morphology of hPSC Aggregates Is Optimal When Using the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels
hPSCs, expanded in suspension using mTeSR™3D in the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels, exhibit the typical morphological characteristics of hPSC aggregates in suspension culture, including a mostly spherical shape, clear edges (not smooth or shiny), an even color, and a dimpled or pock-marked appearance. Aggregates are typically 350-400 µm in size. Scale bar = 500 µm.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
