Caspase-3/7 Activity Plate Reader Assay Kit, Green

For detection of caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis
Catalog #
100-0920_C
For detection of caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis
From: 549 USD

Overview

Efficiently detect caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis by using the Caspase-3/7 Activity Plate Reader Assay Kit, Green, which includes the fluorogenic substrate Z-DEVD-Rh 110-DVED-Z and an assay buffer. This reagent kit uses a quick and simple staining protocol that is well suited for high-throughput screening and compatible with standard fluorescent plate readers.

Caspase-3 and caspase-7 have a proteolytic substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). When added to apoptotic cells, Z-DEVD-Rh 110-DVED-Z is cleaved by activated caspase-3/7, generating a green fluorescent signal that can be detected by standard plate readers.

Caspases are a family of proteases that play a central role in cellular apoptosis through the cleavage of select proteins, and result in the disassembly of the cell (Thornberry & Lazebnik Science, 1998). This process is switched on by pro-apoptotic signals from death receptors or stimuli such as cytotoxic reagents, which activate the initiator caspases and lead to activation of the effector caspases—caspase-3 and caspase-7 (Thornberry & Lazebnik Science, 1998). Caspase assays are useful for quantifying caspase-3/7 activity in apoptotic cells and for screening caspase-3/7 inhibitors.
Advantages
⦁ Z-DEVD-Rh 110-DVED-Z detects caspase-3/7 activity
⦁ Excitation wavelength: 500 nm
⦁ Emission wavelength: 522 nm
⦁ Extinction coefficient: 80,000 cm⁻¹M⁻¹
⦁ Easily scalable for high-throughput assays
⦁ Verified for use with fluorescent plate readers
Components
  • Caspase-3/7 Activity Plate Reader Assay Kit, Green (Catalog #100-0920)
    • Caspase-3/7 Substrate (200X), Green, 2 x 50 µL
    • Assay Buffer, 20 mL
Area of Interest
Cancer
CAS Number
67-68-5

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Caspase-3/7 Activity Plate Reader Assay Kit, Green
Catalog #
100-0920
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Caspase-3/7 Activity Plate Reader Assay Kit, Green
Catalog #
100-0920
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Caspase-3/7 Activity Plate Reader Assay Kit, Green
Catalog #
100-0920
Lot #
All
Language
English

Data and Publications

Data

Figure 1. Caspase-3/7 Activity Plate Reader Assay Kit, Green

Jurkat cells were treated with 0.2% DMSO (Control) or 20 µM camptothecin (CPT), and in the presence or absence of Z-VAD-FMK (a pan-caspase inhibitor). Caspase-3/7 activity was assessed as a measure of fluorescence using the Caspase-3/7 Activity Plate Reader Assay Kit, Green. Data are shown as the mean ± SEM from three independent experiments.

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