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Use PneumaCult™ for Physiologically Relevant Airway Cultures

Air-liquid interface (ALI) culture of human primary airway epithelial cells is being increasingly adopted as a model system for the in vivo human epithelium. Achieving physiologically relevant ALI airway cultures requires carefully considered, optimized culture conditions. Cells expanded with PneumaCult™-EX Plus and differentiated with PneumaCult™-ALI retain key features of the in vivo airway epithelium, such as a pseudostratified, mucociliated epithelial cell layer that comprises goblet cells, basal cells, and motile cilia.